P0020 - Improvement of Blood Glucose and Recovery of Myosin Va Expression in Pancreatic Islets

Pravash R Mishra, MD¹, Abhijit Sahu, MSc², Akansha Das, BSc³, Arun Chaudhury, MD^4
1All India Institute of Medical Sciences, Bhubaneswar, Orissa, India; ²All India Institute of Medical Sciences, Bhubaneshwar, Orissa, India; ³Regional Medical Research, Bhubeneswar, Orissa, India; ⁴Geisinger Wyoming Valley Medical Center, Clarks Summit, PA

Introduction: Diabetes mellitus continues to affect individuals worldwide. Though lifestyle
interventions and effective oral and parenteral agents are available, medication side effects and
“fear of needles” create challenges in clinical scenario. We examine whether plant extracts of
chirayita have the potential to improve diabetes. Use of these herbal product has been
mentioned ion the ancient ayurvedic literature.

Methods: Four sets of rats were used in the present study. All studies were
conducted after obtaining appropriate permissions per institutional guidelines. Alloxan was
used to induce diabetes. Furthermore, hyperglycemia was confirmed using peripheral blood
samples. Diabetic animals were treated with either metformin or hydroalcoholic extract
obtained from the plant S chirayita. After completed treatments pancreatic tissues were
harvested and initially stored in neutral buffered formalin. Tissues that underwent autolytic
changes were not included in the study. Immunohistochemical staining with Myosin Va was
performed with a primary antibody and chromogenic determination with diaminobenzidine.
Islet tissues were examined in the different regions of the pancreas. Image sections were viewed
with ImageJ to quantitate protein expression. Further, Fiji was used to render pseudo coloring
and mapping of cellular expression of Myosin Va.

Results: The studies demonstrates that Myosin Va expression is lost after induction of diabetes
by alloxan in rats, but reversable with metformin and ethnopharmacological agents in chirayita.
Biochemical studies revealed improvement of blood glucose levels with metformin and
chirayita.

Discussion: The result of the present study demonstrates that Myosin Va expression is lost in
chemical diabetes but is reversible with metformin and chirayita extract. Though we have not
directly examined the exocytosis of insulin, the expression of Myosin Va may be inferred as a
surrogate marker of secretary potential of the β-cells of the islets of Langerhans. Improvement
of blood glucose with chirayita may offer adjunct treatment options for type II diabetes mellitus.



Disclosures:


Pravash R Mishra, MD¹, Abhijit Sahu, MSc², Akansha Das, BSc³, Arun Chaudhury, MD⁴. P0020 - Improvement of Blood Glucose and Recovery of Myosin Va Expression in Pancreatic Islets, ACG 2024 Annual Scientific Meeting Abstracts. Philadelphia, PA: American College of Gastroenterology.